Hoahoa whakamatautau
Nga rauemi whakamatautau: miraka hou,xylo-oligosaccharides, Lactobacillus bulgaricus (Lb), Streptococcus thermophilus (St).
Whakahohenga riaka: Whakahohehia te Lactobacillus bulgaricus me te Streptococcus thermophilus i roto i te miraka miraka kia toru nga wa kia pumau o raatau mahi (ka whakatauhia ma te tirotiro moroiti me te miraka miraka), katahi ka penapena ki roto i te pouaka whakamatao hei whakamahi i muri mai. Tikanga whakamatautau: Whakaritea kia 21 nga rahinga rite o nga tauira miraka hou ka wehea kia 3 nga roopu, kia 7 nga tauira mo ia roopu. Tāpirihia ngā kukū (%, w/v) o 0, 0.5, 0.6, 0.7, 0.8 ki ngā tauira o ia rōpū. , 0.9 me te 1.0 xylo-oligosaccharides, ka whakakotahihia te Lb me te St ki nga wahanga o te 2:1, 1:1, me te 1:2 hei hanga i te ahurea timata, katahi ka werohia ki nga roopu e 3 me te nui o te werohanga 3%. Whakawhanahia ki te 42 te mahana tonu°C, me te tango tauira i ia wa ki te ine i te maha o nga huakita ora, te uara pH, te waikawa me te ihirangi waikawa lactic i ia roopu miraka.
hua whakamatautau
(1) Lb: St = 2: 1, te awenga o te xylo-oligosaccharides ki te tipu me te hanga waikawa o nga riaka fermentation miraka.
Ka kitea mai i te Whakaahua 1, 0 ki te 1.5 h ko te wa urutaunga o te riaka me te waahi tuatahi o te urunga ki te wahanga tipu taukohiko. Kare e tere te tipu o te riaka, ka iti haere te uara pH, ka tere te piki o te waikawa me te waikawa lactic. Ina 0.7% te kukū o te xylo-oligosaccharide, ko te tapeke huakita Ko te uara teitei ko 1.94×108 CFU/mL (ko te mana whakahaere he 1.15×108 CFU/mL), a he rite te pH, te waikawa me te waikawa lactic ki te mana whakahaere; i waenganui i te 1.5 haora me te 3.5 haora, ka uru te riaka ki te wahanga tipu taukohiko me te tere tere. Ka tere te heke o te pH, ka tere te piki o te waikawa me te waikawa lactic. I te 0.7% te kukū o te xylo-oligosaccharide, ko te 5.65 te tapeke huakita nui.×108 CFU/mL (ko te mana whakahaere he 5.41×108 CFU/mL). Ko te pH, te waikawa, me te waikawa lactic Ko te 5.82, te 63 me te 4.93, me te pH, te waikawa me te waikawa lactic o te mana he 6.05, 56 me te 4.67. Mai i te 3.5 haora ki te 4.5 haora, ka heke te tapeke o te tatau kitakita, te heke o te pH, me te piki haere o te waikawa me te waikawa lactic.
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